Composite

Part:BBa_K1660009:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)


J23100-B0034-rMpL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 113
    Illegal AgeI site found at 209
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon sequence for E. coli. and add a constitutive promoter J23100 upstream the coding sequence of rMpL, so that this parts can express rMpL lectin continuously.


Source

It comes from Macrolepiota procera cDNA.

References